Berikut hasil FP1 MotoGP Portugal 2021.
| POS | PEMBALAP | TIM | WAKTU/Selisih |
| 1 | Maverick Viñales | Monster Yamaha (YZR-M1) | 1'42.127s |
| 2 | Alex Rins | Suzuki Ecstar (GSX-RR) | +0.151s |
| 3 | Marc Marquez | Repsol Honda (RC213V) | +0.251s |
| 4 | Pol Espargaro | Repsol Honda (RC213V) | +0.255s |
| 5 | Johann Zarco | Pramac Ducati (GP21) | +0.316s |
| 6 | Francesco Bagnaia | Ducati Team (GP21) | +0.337s |
| 7 | Fabio Quartararo | Monster Yamaha (YZR-M1) | +0.401s |
| 8 | Jack Miller | Ducati Team (GP21) | +0.409s |
| 9 | Alex Marquez | LCR Honda (RC213V) | +0.654s |
| 10 | Danilo Petrucci | KTM Tech3 (RC16) | +0.830s |
| 11 | Valentino Rossi | Petronas Yamaha (YZR-M1) | +0.886s |
| 12 | Takaaki Nakagami | LCR Honda (RC213V) | +0.909s |
| 13 | Joan Mir | Suzuki Ecstar (GSX-RR) | +0.933s |
| 14 | Franco Morbidelli | Petronas Yamaha (YZR-M1) | +1.116s |
| 15 | Lorenzo Savadori | Aprilia Gresini (RS-GP)* | +1.412s |
| 16 | Jorge Martin | Pramac Ducati (GP21)* | +1.575s |
| 17 | Miguel Oliveira | Red Bull KTM (RC16) | +1.873s |
| 18 | Luca Marini | Sky VR46 Avintia Ducati (GP19)* | +2.475s |
| 19 | Iker Lecuona | KTM Tech3 (RC16) | +2.840s |
| 20 | Enea Bastianini | Avintia Ducati (GP19)* | +3.198s |
| 21 | Aleix Espargaro | Aprilia Gresini (RS-GP) | +3.208s |
| 22 | Brad Binder | Red Bull KTM (RC16) | +5.647s |
Ket * = rookie