MotoGP Qatar 2021 akan dimulai pukul 21.00 WIB untuk kelas Moto3, kemudian pukul 22.20 WIB untuk kelas Moto2.
Sedangkan kelas raja dari para raja, MotoGP, akan dimulai pukul 00.00 WIB atau Senin (29/3/2021) dini hari WIB.
Berikut starting grid MotoGP 2021 untuk kelas utama, Minggu (28/3/2021).
| Posisi Start | Pembalap | Tim |
| 1 | Francesco Bagnaia | Ducati |
| 2 | Fabio Quartararo | Monster Energy Yamaha |
| 3 | Maverick Vinales | Monster Energy Yamaha |
| 4 | Valentino Rossi | Petronas Yamaha SRT |
| 5 | Jack Miller | Ducati |
| 6 | Johann Zarco | Pramac |
| 7 | Franco Morbidelli | Petronas Yamaha SRT |
| 8 | Aleix Espargaro | Aprilia |
| 9 | Alex Rins | Suzuki Ecstar |
| 10 | Joan Mir | Suzuki Ecstar |
| 11 | Takaaki Nakagami | LCR Honda |
| 12 | Pol Espargaro | Repsol Honda |
| 13 | Enea Bastianini | Esponsorama Racing |
| 14 | Jorge Martin | Pramac |
| 15 | Miguel Oliveira | KTM Red Bull |
| 16 | Alex Marquez | LCR Honda |
| 17 | Stefan Bradl | Repsol Honda |
| 18 | Luca Marini | Esponsorama Racing |
| 19 | Brad Binder | KTM Red Bull |
| 20 | Danilo Petrucci | KTM Tech3 |
| 21 | Iker Lecuona | KTM Tech3 |
| 22 | Lorenzo Savadori | Aprilia |