Pada MotoGP Americas 2021 Aprilia hanya akan diperkuat satu pembalap yakni Aleix Espargaro saja.
Sebab Maverick Vinales memutuskan absen di seri ini lantaran masih dalam keadaan berkabung usai sepupunya Dena Berta Vinales meninggal dunia dalam kecelakaan balapan WSSP300 pada pekan lalu.
Berikut hasil kualifikasi MotoGP Americas 2021 untuk kelas premier.
| POS. | PEMBALAP | TIM | WAKTU/GAP |
| 1 | Francesco Bagnaia | Ducati Team (GP21) | 2'2.781s |
| 2 | Fabio Quartararo | Monster Yamaha (YZR-M1) | +0.348s |
| 3 | Marc Marquez | Repsol Honda (RC213V) | +0.428s |
| 4 | Jorge Martin | Pramac Ducati (GP21)* | +0.497s |
| 5 | Takaaki Nakagami | LCR Honda (RC213V) | +0.511s |
| 6 | Johann Zarco | Pramac Ducati (GP21) | +0.598s |
| 7 | Alex Rins | Suzuki Ecstar (GSX-RR) | +0.672s |
| 8 | Joan Mir | Suzuki Ecstar (GSX-RR) | +0.747s |
| 9 | Luca Marini | Sky VR46 Avintia Ducati (GP19)* | +0.765s |
| 10 | Jack Miller | Ducati Team (GP21) | +0.939s |
| 11 | Brad Binder | Red Bull KTM (RC16) | +1.000s |
| 12 | Pol Espargaro | Repsol Honda (RC213V) | +1.094s |
| 13 | Franco Morbidelli | Monster Yamaha (YZR-M1) | 2'3.872s |
| 14 | Andrea Dovizioso | Petronas Yamaha (YZR-M1) | 2'4.044s |
| 15 | Alex Marquez | LCR Honda (RC213V) | 2'4.1s |
| 16 | Enea Bastianini | Avintia Ducati (GP19)* | 2'4.118s |
| 17 | Iker Lecuona | KTM Tech3 (RC16) | 2'4.324s |
| 18 | Miguel Oliveira | Red Bull KTM (RC16) | 2'4.392s |
| 19 | Aleix Espargaro | Aprilia Gresini (RS-GP) | 2'4.419s |
| 20 | Valentino Rossi | Petronas Yamaha (YZR-M1) | 2'4.699s |
| 21 | Danilo Petrucci | KTM Tech3 (RC16) | 2'4.829s |